Novel LC-Orbitrap Method for Quantifying De Novo Lipogenesis: Applications in Nutritional States in SD Rats

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June 12,2026

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Measurement of DNL can provide insights into mechanisms and guide interventions if performed rapidly and noninvasively. DNL is calculated based on the measurement of newly synthesized palmitic acid and body water, typically using isotope tracers. The current method for determining DNL employs GC-Orbitrap, which offers superior isotope resolution but has limitations in instrument availability. In this study, we developed a fast, accurate, and convenient method for DNL determination using LC-Orbitrap, expanding the technical approaches available for DNL detection effectively.

Novel-LC-Orbitrap-Method-for-Quantifying-De-Novo-Lipogenesis-Applications-in-Nutritional-States-in-SD-Rats.jpg

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