In Vivo MetID (Metabolite Profiling and Identification)

Result: Figure 1 are metabolite profiling and ID results of pooled human plasma, urine and feces using LC/HRMS. The results indicate that the parent drug is the major pharmacological component exposed, while multiple minor metabolites are present in plasma. Metabolite M3 and M4 (glucuronidation) and M7 (mono-oxygenation metabolite) are most likely the major metabolites in urine and feces, suggesting that the formation of M3/M4 and M7 may be major metabolic clearance pathways in human. Several minor metabolic pathways lead to minor metabolites in urine and feces. The parent drug is a major drug-related component in feces, which may be unabsorbed, excreted into bile and/or from hydrolysis of M3 and M4 and other glucuronides in feces. The parent drug is a minor drug-related component in urine, suggesting that direct excretion of the parent drug from kidney to urine is not a major clearance pathway.