High Throughput LC-MS/MS Methods for Determination of Metabolites from Eight Key Probe Substrates in CYP Time-Dependent Inhibition Assays

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July 17,2026

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The ADDA-LC-MS/MS employed multiplexed LC technology, achieving two‑fold faster sample throughput versus conventional LC without compromising chromatographic separation. Excellent linearity was observed over the validated concentration ranges (r > 0.99 for all metabolites). Intra‑day accuracy was within ±20% (mean accuracy 80–120%) and precision (RSD) was ≤25%.

High Throughput LC-MSMS Methods for Determination of Metabolites from Eight Key Probe Substrates in CYP Time-Dependent Inhibition Assays.jpg

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