Establishment of a Quantitative Analytical Method for Albumin-Drug Conjugates in Rat Plasma Using Ultra-high Performance Liquid Chromatography-Tandem Mass Spectrometry

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March 17,2026

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Albumin is present in very high concentration in plasma. In the literature, anti-albumin magnetic beads or anti-albumin antibodies were used for enrichment, which was expensive and required a large number of magnetic beads for each sample. This study has developed a high-throughput cold acetone extraction method, which greatly simplified the pretreatment process and shortened the pretreatment time. It is a cost-effective, efficient, and suitable method for the analysis of large-scale biological samples.

Establishment of a Quantitative Analytical Method for Albumin-Drug Conjugates in Rat Plasma Using Ultra-high Performance Liquid Chromatography-Tandem Mass Spectrometry

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