A Novel RT-qPCR Methodology for Quantitative Bioanalysis of Total and Conjugated siRNA in Antibody-Oligonucleotide Conjugates (AOCs)

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April 17,2026

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This study presents a novel RT-qPCR methodology specifically developed for antibody-siRNA conjugate quantification, significantly advancing AOC research and development. The optimized qPCR protocol was rigorously validated for essential performance parameters, including linearity, sensitivity, amplification efficiency, and specificity.

A Novel RT-qPCR Methodology for Quantitative Bioanalysis of Total and Conjugated siRNA in Antibody-Oligonucleotide Conjugates (AOCs)

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