One-stop Metabolite Biosynthesis and Structural Characterization

The precise structures of most metabolites are often challenging to predict based solely on high-resolution mass spectrometry (HRMS) data during in vitro or in vivo metabolite identification (MetID). This challenge leads to hurdles in achieving targeted synthesis of the desired metabolites using traditional chemical methods. Consequently, the chemical synthesis process becomes time-consuming, labor-intensive, and intricate, with a potential for mismatches. In addition, other metabolites are difficult to synthesize by chemical method due to the stability, configuration or long synthetic route issues, etc. Metabolite biosynthesis, which is performed by using biotransformation system of in vitro incubation and in vivo animal administration, is a good choice to solve these problems. Utilizing preparative liquid chromatography (LC), high-resolution mass spectrometry (HRMS), and nuclear magnetic resonance (NMR) identification technology, the WuXi AppTec DMPK has established a biosynthesis platform for targeted metabolites. This platform can efficiently synthesize metabolites at milligram (mg) level and accurately identify their precise structures.