Investigation Strategies of the ADC Payload with Thiol Group in In Vivo Samples
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Posters
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May 30,2026
Posters Overview
After ADC payload or payload-linker is cleaved in vivo by enzymes, for the payloads bearing thiol groups, they will not exist simply as “conjugated to antibody” or “free” species. Instead, they can be present in multiple forms due to the disulfide bond: the free payload, payload–protein adducts, payload dimers, and payload–cysteine adducts. Determining what payload species are present in target tissues and the concentration of each is critical for understanding the actual effect of the designed ADC and guiding its clinical use. In this study, experiments were designed to characterize payload–protein adducts and to identify and accurately quantify each species using LC–MS/MS and LC–HRMS.
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