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The ferret (Mustela putorius furo) is an indispensable experimental animal model in the fields of influenza virology, respiratory diseases, and vaccine development. However, its unique physiological and anatomical characteristics pose significant challenges for venous blood sampling. The peripheral blood vessels, such as the saphenous and caudal veins, are extremely narrow (0.3-0.5mm in diameter), and the subcutaneous fat layer combined with thick epidermal structures complicates visualization. Additionally, ferrets exhibit a more pronounced stress-induced vascular contraction response compared to rodents, resulting in a lower success rate of traditional blood sampling.

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