Bioanalytical Strategy for Antibody-Drug Conjugates (ADCs) Based on Integrated Mass Spectrometry Bioanalysis Platform

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September 11,2025

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In this study, we summarised a comprehensive bioanalytical strategy of ADCs based on mass spectrometry platforms, including tandem mass spectrometry (MS/MS) and high-resolution mass spectrometry (HRMS). Including measurement for total antibody, conjugated antibody, free payload, conjugated payload, total payload, drug-to-antibody ratio (DAR), and biotransformation. A deep understanding of these contents is key to characterising ADC behaviour through drug discovery to development stages.

Bioanalytical Strategy for Antibody-Drug Conjugates (ADCs) Based on Integrated Mass Spectrometry Bioanalysis Platform

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