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Understanding and characterizing the major released payload-related species is crucial for supporting lead optimization, bioanalytical method development, and ADME and DDI studies of ADCs.The objective of this study was to establish a quadrupole-orbitrap HRMS-based workflow for rapid screening and characterizing the payload-related species released from ADC after incubating with in vitro plasma, S9 fraction, lysosome, and tumor cells.
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