Is the Direct or Indirect Method the Ideal One for Evaluation of Blood to Plasma Ratio?

Posters
April 29,2025

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In vitro blood to plasma ratio (BPR) assays are important for understanding drug distribution into red blood cells (RBCs), thus informing a rational choice of appropriate biological fluid for pharmacokinetic analysis. Two methods, namely the direct method and the indirect method, are routinely performed in the industry. In the direct method, analyte concentrations in blood and plasma separated from blood are analyzed, while in the indirect method, analyte concentrations in plasma separated from blood, as well as an incubated plasma, are determined.

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