Probing Drug and Metabolite Covalent Binding with a Non-Radiolabeled Method: Osimertinib as an Example
-
Posters
-
April 29,2025
Posters Overview
In this study, a novel LC-MS approach using targeted Precursor Ion Scan to identify protein-conjugated drug-related compounds was developed and optimized. After organic solvent extraction, the resulting residue of the incubated sample undergoes proteolytic digestion. We utilize non-targeted scanning for multiple fragment ions of the parent compound to identify products where the parent compound or its metabolites are covalently bound to amino acids or peptides. Using osimertinib as a model compound, we first conducted a conventional MetID study with the supernatant, followed by a MetID analysis of the residue after extraction. We investigated multiple matrices, including plasma and human serum albumin (HSA).
Complete the form to view and download this poster.
Stay Connected
Keep up with the latest news and insights.
