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Nucleoside-based drugs, recognized as purine or pyrimidine analogs, are potent therapeutic agents specifically used for the treatment of viral infections. These drugs exhibit complex interactions with cellular molecular constituents, primarily via activation of phosphorylation cascades leading to consequential interactions with nucleic acids. Determination of nucleoside mono-, di- and triphosphates (NMP, NDP, and NTP) and their deoxy-counterparts (dNMP, dNDP, dNTP), can intuitively reflect the mechanisms of action and efficacy of these drugs.
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