A Separation Method of D-(-)-Fructose, Glucose and Galactose in C57BL/6J Mouse Plasma by LC-MS/MS

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June 07,2023

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Monosaccharides are endogenous components widely present in biologicalmatrices. They are often applied as biomarkers to indicate disease occurrence. The separation and determination of D-(-)-fructose from its isomers such as glucose and galactose is a great challenge as they have the same molecular composition, similar structures. Moreover, all of these isomers exhibit poor chromatography retention due to their high hydrophilicity. In this study, a validated, rapid, and reproducible LC-MS/MS method was developed for the quantitative determination of D-(-)-fructose in a pharmacokinetics study.

117A Separation Method of D-(-)-Fructose.jpg

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