AAPS 2025 PHarmSCi 360

Biocytogen humanized neonatal Fc receptor mice (B-hFcRn mice) offer an alternative model for Tg32 mice in pharmacokinetic studies of biologics; however, differences between B-hFcRn and Tg32 mice raise uncertainties about whether data from B-hFcRn mice can reliably predict human pharmacokinetics.

The ferret (Mustela putorius furo) is an indispensable experimental animal model in the fields of influenza virology, respiratory diseases, and vaccine development. However, its unique physiological and anatomical characteristics pose significant challenges for venous blood sampling. The peripheral blood vessels, such as the saphenous and caudal veins, are extremely narrow (0.3-0.5mm in diameter), and the subcutaneous fat layer combined with thick epidermal structures complicates visualization. Additionally, ferrets exhibit a more pronounced stress-induced vascular contraction response compared to rodents, resulting in a lower success rate of traditional blood sampling.

Ball milling technology has the potential to enhance the oral bioavailability of poorly soluble drugs by reducing particle size, which can lead to improved systemic exposure in vivo. For heat-sensitive drugs, it is important to use intermittent milling and controlled speed to prevent degradation. We have evaluated a series of dispersants to ensure stability and proper particle size for nanometer suspension. Selecting the appropriate stabilizer and carefully considering critical instrument parameters are crucial steps in preparing effective nanometer suspensions. In summary, the careful selection of dispersants and the optimization of milling parameters are essential for preparing high-performance nanometer suspensions.